Tim Farron stressed that his religion was a "personal matter" but admitted it was "odd" to suggest his politics would not be influenced by God.
Mr Farron said the main influence that his Christianity had on his politics was a belief that "everybody is equal".
Speaking to 5 News he dismissed suggestions secular voters could be wary of backing him because of his faith.
Asked if he prayed for guidance before taking decisions, he told the broadcaster: "I think you would be surprised if any person who was religious didn't pray from time to time.
"My faith is a personal matter to me. Of course there are people of all faiths and none, and we are a party who famously champion religious tolerance and demanding rights and freedom for those people who belong to minorities.
"The party got set up in the 1860s by non-conformists, by atheists, by Catholics, by Jewish people who were against the overweening power of the Church of England. I come from that kind of a background."
Asked if people would have concerns about voting for someone so religious, he replied: "I would be very surprised. I think if you are somebody who rams your faith down other people's throats, or even the kind of person who demands that people shouldn't have a faith, then people would suspect that kind of extreme position.
"But we are a tolerant race and we believe that people should be entitled to have a faith or, indeed, no faith."
Meanwhile on Sky News he admitted it would be "a really odd thing to think that somebody who had a faith wasn't ever affected by it".
He accepted his Christian belief influenced his decision to reject the Assisted Dying Bill.
"But my faith tells me, above all else, that everybody is of equal value and everybody is equal. We should, for example, support the right to equal marriage, people should be free to love who they love and we should have a tolerant society," he said.