It is part of Bible Society's "Pass it On" campaign, designed to answer the call from parents and young children to keep Bible stories alive for future generations.
Travel is fundamental to the Christmas story, where Mary and Joseph travel from Nazareth to Bethlehem and the Shepherds from their fields as well as the wise men from the East.
Rachel Rounds from the Bible Society explained why they decided to create the tube map: "On our research we found that a third of children didn't know the nativity was a story from the Bible.
"We think that's a real shame - so we want to keep the story alive and think that using the internet is a great way of reaching a younger audience.
"When it pops up, you'll see some tube doors open, and it starts at Angel tube, which was thought was an appropaite place to start."
She said they chose it for its easily reconginisable features: "The London tube map, it's so iconic isn't it? Everybody looks at it and throughout the world everybody knows it.
"It's that sort of sense of travel really, and this weekend will be the busiest travel weekend for everybody in cars, trains, whatever."
The idea is that children and adults can interact with the map and follow the nativity story, navigating through each part: "Wouldn't it be fun to have a nativity based on a tube map - so we've renamed some of the well-known London stations with a nativity-pun if you like - so Hammersmith is Herod-Smith, Bethnal Green is Bethlemhem Green, and Westminster is Wise-menster."
Listen to Premier's Hannah Tooley speak to Rachel Rounds here: