Campaigners from the British Pregnancy Advisory Service demanded a price cut after they claimed that British women pay up to five times more than their European neighbours.
Rivals Superdrug and Tesco have halved their prices but the chief pharmacist for Boots, Marc Donovan said that they do not want to be "accused of incentivising inappropriate use".
Alethea Williams, from the Society for the Protection of Unborn Children (SPUC) told Premier she agreed.
During News Hour, Williams - who is a Christian - said: "This is a serious drug which all advice says should not be used frequently.
"So the fact that Boots are not giving into pressure to make it more readily available - while they are willing to sell it which is not wonderful, at least they're showing some sort of responsibility on this."
Currently, the progestogen-based drug Levonelle costs £28.25 in Boots, and its non-branded equivalent is £26.75.
But the branded drug costs £13.50 at Tesco and a generic version is £13.49 in Superdrug.
However, Superdrug charges £27 for Levonelle and £35 for an alternative emergency contraceptive pill, EllaOne.
When asked to explain their stance, Boots released a statement saying the price of emergency contraception included "a professional healthcare consultation".
It said: "This consultation helps support customers in their choice by examining an individual's full medical history and any potential drug interactions."
Listen to Alethea Williams, from SPUC speaking with Premier's Eno Adeogun:
