Recently passed legislation which changes the states anti-discrimination law to include gender identity will come into effect in October.
The Massachusetts Commission Against Discrimination, which is responsible for enforcing the law, had published new guidance which sets out the legal requirements of employers as well as public spaces.
The law, which has been endorsed by President Barak Obama, has received criticism from some religious leaders in the USA.
In May, a group of Catholic bishops spoke out, calling toilets based on gender identity rather than biological sex "disturbing".
Bishop Richard Malone of Buffalo and Archbishop George Lucas, who are both committee chair people of the US Conference of Catholic Bishops, issued a statement saying: "The guidance fails to address a number of important concerns and contradicts a basic understanding of human formation so well expressed by Pope Francis: that 'the young need to be helped to accept their own body as it was created.'"
The guidance says public accommodation is "any place, whether licensed or unlicensed, which is open to and accepts or solicits the patronage of the general public."
The law applies to public swimming pools, cinemas and shopping malls.
The Daily Caller has reported that church leaders are concerned they will be made to comply with the new legislation.
The guidelines say: "Even a church could be seen as a place of public accommodation if it holds a secular event, such as a spaghetti supper, that is open to the general public."
The suggested rules also ask that employers are respectful and call employees by their preferred name and pronouns.
